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Positive WB detected in：rat spleen tissue, mouse spleen tissue
Positive IP detected in：——
Positive IHC detected in：human breast cancer tissue, human colon cancer tissue, human liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in：——
Positive FC (Intra) detected in：HepG2 cells
推荐稀释比：应用 推荐稀释比
Western Blot (WB) WB : 1:200-1:1000
Immunohistochemistry (IHC) IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA) FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent.
免疫原：Domain: 167-261 aa of BC000938
Sequence: LRLRAPLQQVQAQLSPLQNISPWILAVLTLQIQSLISCWAFWTTWTQSCSSNALPQSLPAWRSSQRSTQKDPVPYQPPFLCQWGRHQPSWKPLMN
抗体类别：Polyclonal
产品类型：Antibody
全称：X-box binding protein 1
计算分子量：261 aa, 29 kDa
观测分子量：32 kDa
GenBank蛋白编号：BC000938
基因名称：XBP1
Gene ID (NCBI)：7494
偶联类型：Unconjugated
形式：Liquid
纯化方式：Antigen affinity purification
UNIPROT ID：«UNIPROT ID»
储存缓冲液：PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件：Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.
背景介绍：X-box-binding protein 1 (XBP1), also named as TREB5, is a 261 amino acid protein, which contains one bZIP Domain and belongs to the bZIP family. XBP1 localizes in the nucleus and as a transcription factor is essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response. XBP1 has an association with major affective disorder. The molecular weight of protein generated from the spliced XBP1 mRNA is 54 kDa and protein generated from unspliced Xbp1 mRNA is 33 kDa. This antibody 25997-1-AP specially recognizes isoform XBP-1U.
注意事项
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