HyCyte® | 海星生物

Positive WB detected in：HEK-293 cells, HSC-T6 cells, HeLa cells, Jurkat cells, L02 cells, PC-12 cells

Positive IP detected in：——

Positive IHC detected in：mouse colon tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF/ICC detected in：HEK-293 cells

Positive FC (Intra) detected in：——

推荐稀释比：应用 推荐稀释比

Western Blot (WB) WB : 1:500-1:3000

Immunohistochemistry (IHC) IHC : 1:1000-1:4000

Immunofluorescence (IF)/ICC IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent.

免疫原：Domain: 1-95 aa of BC016775

Sequence: MADEKPKEGVKTENNDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGGVY

抗体类别：Polyclonal

产品类型：Antibody

全称：SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)

计算分子量：11 kDa

观测分子量：11-20 kDa

GenBank蛋白编号：BC016775

基因名称：SUMO2

Gene ID (NCBI)：6613

偶联类型：Unconjugated

形式：Liquid

纯化方式：Antigen affinity purification

UNIPROT ID：«UNIPROT ID»

储存缓冲液：PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

储存条件：Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

背景介绍：Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.

注意事项

1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗等用途。

2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。