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Positive WB detected in：BxPC-3 cells, HEK-293 cells, NIH/3T3 cells, MCF-7 cells
Positive IP detected in：——
Positive IHC detected in：——
Positive IF/ICC detected in：HepG2 cells
Positive FC (Intra) detected in：HepG2 cells
推荐稀释比：应用 推荐稀释比
Western Blot (WB) WB : 1:5000-1:50000
Immunofluorescence (IF)/ICC IF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA) FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
CHIP-QPCR CHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent.
免疫原：Domain: 13-227 aa of BC002379
Sequence: NDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPSSMMVKDEYVHDFEGQPSLSTEGHSIQTIQHPPSNRASTETYSTPALLAPSESNATSTANFPNIPVASTSQPAS
抗体类别：Recombinant
产品类型：Antibody
全称：SMAD family member 4
计算分子量：60 kDa
观测分子量：63-70 kDa
GenBank蛋白编号：BC002379
基因名称：SMAD4
Gene ID (NCBI)：4089
偶联类型：Unconjugated
形式：Liquid
纯化方式：Protein A purfication
UNIPROT ID：«UNIPROT ID»
储存缓冲液：PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件：Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.
背景介绍：Mammalian homologs of the Drosophila Mad gene include Smad1, Smad2, Smad3, Smad4 (DPC4), Smad5, Smad6, Smad7 and Smad8. Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/ TGF signaling by interfering with TGF-mediated phosphorylation of other Smad family members.
注意事项
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗等用途。
2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。