Positive WB detected in:HeLa cells, A549 cells, Raji cells
Positive IP detected in:——
Positive IHC detected in:human tonsillitis tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in:——
Positive FC (Intra) detected in:——
推荐稀释比:应用 推荐稀释比
Western Blot (WB) WB : 1:500-1:2000
Immunohistochemistry (IHC) IHC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent.
免疫原:Domain: 1-310 aa of BC008765
Sequence: MRRAALWLWLCALALSLQPALPQIVATNLPPEDQDGSGDDSDNFSGSGAGALQDITLSQQTPSTWKDTQLLTAIPTSPEPTGLEATAASTSTLPAGEGPKEGEAVVLPEVEPGLTAREQEATPRPRETTQLPTTHQASTTTATTAQEPATSHPHRDMQPGHHETSTPAGPSQADLHTPHTEDGGPSATERAAEDGASSQLPAAEGSGEQDFTFETSGENTAVVAVEPDRRNQSPVDQGATGASQGLLDRKEVLGGVIAVGLVGLIFAVCLVGFMLYRMKKKDEGSYSLEEPKQANGGAYQKPTKQEEFYA
抗体类别:Polyclonal
产品类型:Antibody
全称:syndecan 1
计算分子量:32.5 kDa
观测分子量:60-100 kDa, 130 kDa
GenBank蛋白编号:BC008765
基因名称:CD138
Gene ID (NCBI):6382
偶联类型:Unconjugated
形式:Liquid
纯化方式:Antigen affinity purification
UNIPROT ID:«UNIPROT ID»
储存缓冲液:PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件:Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.
背景介绍:CD138, also named as Syndecan-1 (SDC1), is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. CD138 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas. CD138 has a calculated molecular weight of 32.5 kDa, the apparent molecular weight is higher due to extensive glycosylation.
注意事项
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗等用途。
2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。