Positive WB detected in:K-562 cells, NIH-3T3 cells (RNAi)
Positive IP detected in:K-562 cells
Positive IHC detected in:human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in:C6 cells
Positive FC (Intra) detected in:——
推荐稀释比:应用 推荐稀释比
Western Blot (WB) WB : 1:500-1:1000
Immunoprecipitation (IP) IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC) IHC : 1:50-1:500
Immunofluorescence (IF)/ICC IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent.
免疫原:Domain: 449-801 aa of BC040300
Sequence: IGELQVELPEVHTNSCDNISQFSVDSITSQESKEPVFIAAGDIRRRLSEQLAHTPTAFKRDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYKILVISADSGMIEPVVNAVSIHQVKKQSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSPRNLGFETSAFKLTTEFVDVMGGLDGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGSQLPCFHGSSTIRNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGIM
抗体类别:Polyclonal
产品类型:Antibody
全称:phosphatidylinositol 4-kinase, catalytic, beta
计算分子量:801 aa, 90 kDa
观测分子量:100 kDa
GenBank蛋白编号:BC040300
基因名称:PI4KB
Gene ID (NCBI):5298
偶联类型:Unconjugated
形式:Liquid
纯化方式:Antigen affinity purification
UNIPROT ID:«UNIPROT ID»
储存缓冲液:PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件:Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.
背景介绍:PI4KB(phosphatidylinositol 4-kinase beta) is also named as PIK4CB, NPIK, PI4K92 and belongs to the PI3/PI4-kinase family. It may regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation and involved in Golgi-to-plasma membrane trafficking. PI4KB is a type III enzyme that is sensitive to wortmannin(PMID:9020160). This protein has 3 isoforms produced by alternative splicing.
注意事项
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗等用途。
2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。