Positive WB detected in:mouse kidney tissue, HeLa cells, rat liver tissue, HepG2 cells, PC-12 ceslls
Positive IP detected in:L02 cells
Positive IHC detected in:mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in:A549 cells
Positive FC (Intra) detected in:——
推荐稀释比:应用 推荐稀释比
Western Blot (WB) WB : 1:1000-1:8000
Immunoprecipitation (IP) IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC) IHC : 1:50-1:500
Immunofluorescence (IF)/ICC IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent.
免疫原:Domain: 1-373 aa of BC023601
Sequence: MGSQVSVESGALHVVIVGGGFGGIAAASQLQALNVPFMLVDMKDSFHHNVAALRASVETGFAKKTFISYSVTFKDNFRQGLVVGIDLKNQMVLLQGGEALPFSHLILATGSTGPFPGKFNEVSSQQAAIQAYEDMVRQVQRSRFIVVVGGGSAGVEMAAEIKTEYPEKEVTLIHSQVALADKELLPSVRQEVKEILLRKGVQLLLSERVSNLEELPLNEYREYIKVQTDKGTEVATNLVILCTGIKINSSAYRKAFESRLASSGALRVNEHLQVEGHSNVYAIGDCADVRTPKMAYLAGLHANIAVANIVNSVKQRPLQAYKPGALTFLLSMGRNDGVGQISGFYVGRLMVRLTKSRDLFVSTSWKTMRQSPP
抗体类别:Polyclonal
产品类型:Antibody
全称:apoptosis-inducing factor, mitochondrion-associated, 2
计算分子量:41 kDa
观测分子量:41 kDa
GenBank蛋白编号:BC023601
基因名称:AIFM2/ FSP1
Gene ID (NCBI):84883
偶联类型:Unconjugated
形式:Liquid
纯化方式:Antigen affinity purification
UNIPROT ID:«UNIPROT ID»
储存缓冲液:PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件:Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.
背景介绍:The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis-associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis-Inducing Factor), a mitochondrion-associated enzyme that relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.
注意事项
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗等用途。
2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。