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Positive WB detected in：HeLa cells, mouse heart tissue, mouse testis tissue, mouse lung tissue, mouse liver tissue, SH-SY5Y cells, mouse brain tissue, mouse ovary tissue, HepG2 cells, rat brain tissue
Positive IP detected in：——
Positive IHC detected in：human heart tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in：HepG2 cells
Positive FC (Intra) detected in：——
推荐稀释比：应用 推荐稀释比
Western Blot (WB) WB : 1:500-1:2000
Immunohistochemistry (IHC) IHC : 1:20-1:200
Immunofluorescence (IF)/ICC IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent.
免疫原：Peptide
抗体类别：Polyclonal
产品类型：Antibody
全称：kelch-like 14 (Drosophila)
计算分子量：70 kDa
观测分子量：39-45 kDa
GenBank蛋白编号：NM_020805
基因名称：KLHL14
Gene ID (NCBI)：57565
偶联类型：Unconjugated
形式：Liquid
纯化方式：Antigen affinity purification
UNIPROT ID：«UNIPROT ID»
储存缓冲液：PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件：Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.
背景介绍：KLHL14, a member of the Kelch-like gene family, has six Kelch Domains and is localized to the endoplasmic reticulum. KLHL14, a novel tumor suppressor, is a subunit of a ubiquitin ligase that associates with the endoplasmic reticulum-associated protein degradation (ERAD) machinery, regulating of B cell receptor-dependent NF-κB signaling (PMID: 32127472). KLHL14 has two isoforms of 70kDa and 43kDa.
注意事项
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗等用途。
2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。