HyCyte® | 海星生物

Positive WB detected in：human testis tissue, human skeletal muscle tissue

Positive IP detected in：——

Positive IHC detected in：human small intestine tissue, human colon tissue, human colon cancer tissue, human kidney tissue, human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF/ICC detected in：——

Positive FC (Intra) detected in：——

推荐稀释比：应用 推荐稀释比

Western Blot (WB) WB : 1:2000-1:6000

Immunohistochemistry (IHC) IHC : 1:1000-1:4000

Immunofluorescence (IF)-P IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

文献引用反应性：Human, Mouse, Monkey

免疫原：CatNo: Ag15554

Product name: Recombinant human ACE2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 392-744 aa of BC048094

Sequence: LRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVSIWLI

抗体类别：Monoclonal

产品类型：Antibody

全称：angiotensin I converting enzyme (peptidyl-dipeptidase A) 2

计算分子量：805 aa, 92 kDa

观测分子量：120 kDa, 92 kDa

GenBank蛋白编号：BC048094

基因名称：ACE2

Gene ID (NCBI)：59272

RRID：AB_2882052

偶联类型：Unconjugated

形式：Liquid

纯化方式：Protein G purification

UNIPROT ID：Q9BYF1

储存缓冲液：PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

储存条件：Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍：ACE2 (Angiotensin-converting enzyme 2), also named as ACEH, is a zinc metalloprotease of the ACE family and a critical regulator of the reninangiotensin system. ACE2 has a more restricted tissue distribution than ACE, being found predominantly in the heart, kidneys, and testes although low levels have been detected in a variety of tissues (PMID:15983030). ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (PMID: 32142651). The expression level and expression pattern of human ACE2 in different tissues might be critical for the susceptibility, symptoms, and outcome of 2019-nCoV/SARS-CoV-2 infection (PMID: 32133153). It can be used as a potential therapeutic target of SARS-CoV-2 (PMID: 32125455). The calculated molecular weight of ACE2 is 92kDa but it migrates to 120kDa due to N-glycosylation (PMID:16166094). Sometimes, several cleaved fragments can also be detected as 75kDa, 50 kDa or 37kDa (PMID: 29561187, 22009550, 30759273). It has 2 isoforms produced by alternative splicing. This antibody is specific to ACE2. The location of ACE2 is membrane and cytoplasm, however it accumulates in the nucleus during the mitosis (PMID: 1730413/PMID: 18292088).

注意事项

1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗等用途。

2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。