HyCyte® | 海星生物

Positive WB detected in：Jurkat cells, HL-60 cells, K-562 cells

Positive IP detected in：——

Positive IHC detected in：human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF/ICC detected in：——

Positive FC (Intra) detected in：——

推荐稀释比：应用 推荐稀释比

Western Blot (WB) WB : 1:1000-1:6000

Immunohistochemistry (IHC) IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

文献引用反应性：Human

免疫原：CatNo: Ag14254

Product name: Recombinant human ACAP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 20-251 aa of BC018543

Sequence: SIELVEAEVSELETRLEKLLKLGTGLLESGRHYLAASRAFVVGICDLARLGPPEPMMAECLEKFTVSLNHKLDSHAELLDATQHTLQQQIQTLVKEGLRGFREARRDFWRGAESLEAALTHNAEVPRRRAQEAEEAGAALRTARAGYRGRALDYALQINVIEDKRKFDIMEFVLRLVEAQATHFQQGHEELSRLSQYRKELGAQLHQLVLNSAREKRDMEQRHVLLKQKELG

抗体类别：Monoclonal

产品类型：Antibody

全称：ArfGAP with coiled-coil, ankyrin repeat and PH domains 1

计算分子量：740 aa, 82 kDa

观测分子量：81 kDa

GenBank蛋白编号：BC018543

基因名称：ACAP1

Gene ID (NCBI)：9744

RRID：AB_2881956

偶联类型：Unconjugated

形式：Liquid

纯化方式：Protein G purification

UNIPROT ID：Q15027

储存缓冲液：PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

储存条件：Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍：ACAP1, also named as Centaurin-beta-1, is a 740 amino acid protein, which is expressed Highly in lung and spleen. ACAP1 as a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface and required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.

注意事项

1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗等用途。

2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。