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Positive WB detected in：K-562 cells, HCT 116 cells, K562, NCI-H1299 cells
Positive IP detected in：——
Positive IHC detected in：human pancreas cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in：HeLa cells
Positive FC (Intra) detected in：——
推荐稀释比：应用推荐稀释比
Western Blot (WB) WB : 1:1000-1:4000
Immunohistochemistry (IHC) IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
文献引用反应性：Human, Chicken
免疫原：CatNo: Ag18985
Product name: Recombinant human 4EBP1 protein
Source: e coli.-derived, PET28a
Tag: 6*His
Domain: 1-118 aa of BC004459
Sequence: MSGGSSCSQTPSRAIPATRRVVLGDGVQLPPGDYSTTPGGTLFSTTPGGTRIIYDRKFLMECRNSPVTKTPPRDLPTIPGVTSPSSDEPPMEASQSHLRNSPEDKRAGGEESQFEMDI
抗体类别：Monoclonal
产品类型：Antibody
全称：eukaryotic translation initiation factor 4E binding protein 1
计算分子量：118 aa, 12 kDa
观测分子量：15-24 kDa
GenBank蛋白编号：BC004459
基因名称：EIF4EBP1
Gene ID (NCBI)：1978
RRID：AB_2881368
偶联类型：Unconjugated
形式：Liquid
纯化方式：Protein A purification
UNIPROT ID：Q13541
储存缓冲液：PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件：Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
背景介绍：Eukaryotic translation initiation factor 4E-binding protein 1(4EBP1), is a member of 4EBPs family, which regulate the translation of a subset of mRNA by competing with eIF4G for binding to eIF4E, thus preventing the assembly of the eIF4F complex. The eIF4F facilitates the recruitment of other translation initiation factors to form the complex and then initiates cap-dependent translation.4EBP1 also mediates the regulation of protein translation by growth factors, hormones and other stimuli that signal through the MAP kinase and mTORC1 pathways. There are three forms of 4EBP1, alpha, beta and gamma.typical pattern seen on Western blots for phosphorylated heat-acid stabled protein 4EBP1 from rat gastrocnemius muscle. Three distinct bands are noted, with the most rapidly migrating band having an apparent molecular mass of ; 20 kDa and the slowest migrating band of; 24 kDa. The 3 bands are designated by convention as alpha, beta and gamma. (PMID: 10913029)
注意事项
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗等用途。
2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。