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Positive WB detected in：HeLa cells, mouse liver tissue, mouse brain tissue, Jurkat cells, rat liver tissue, mouse heart tissue, NIH/3T3 cells, C6 cells
Positive IP detected in：mouse lung tissue
Positive IHC detected in：——
Positive IF/ICC detected in：HepG2 cells
Positive FC (Intra) detected in：HeLa cells
推荐稀释比："应用 推荐稀释比Western Blot (WB) WB : 1:1000-1:4000
Immunoprecipitation (IP) IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA) FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery."
文献引用反应性：Human, Mouse, Rat
免疫原：
CatNo: Ag5959
Product name: Recombinant human 14-3-3 protein
Source: e coli.-derived, PGEX-4T
Tag: GST
Domain: 1-245 aa of BC056867
Sequence: MEKTELIQKAKLAEQAERYDDMATCMKAVTEQGAELSNEERNLLSVAYKNVVGGRRSAWRVISSIEQKTDTSDKKLQLIKDYREKVESELRSICTTVLELLDKYLIANATNPESKVFYLKMKGDYFRYLAEVACGDDRKQTIDNSQGAYQEAFDISKKEMQPTHPIRLGLALNFSVFYYEILNNPELACTLAKTAFDEAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDSAGEECDAAEGAEN"
抗体类别：Polyclonal
产品类型：Antibody
全称：tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
计算分子量：28 kDa
观测分子量：31 kDa
GenBank蛋白编号：BC056867
基因名称：14-3-3 theta
Gene ID (NCBI)：10971
RRID：AB_2218096
偶联类型：Unconjugated
形式：Liquid
纯化方式：Antigen affinity purification
UNIPROT ID：P27348
储存缓冲液：PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件：Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
背景介绍：14-3-3 proteins are the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.
注意事项
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗等用途。
2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。