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细胞名称：Cas9 标记的人乳腺癌细胞
细胞简称：MDA-MB-231-Cas9
产品货号：TCH-C453C
转导方法：慢病毒法
抗性基因：Hygro
荧光基因：—
克隆类型：Poolcells
靶细胞名称：人乳腺癌细胞
靶细胞简称：MDA-MB-231
种属来源：人
组织来源：乳腺
疾病特征：乳腺癌
细胞形态：上皮细胞样
生长特性：贴壁生长
培养体系：DMEM-H+10%FBS+2000.0 ug/mL Hygromycin+1%P/S
配套培养基货号：TCH-G453C
传代比例：1:3-1:6，每2-3天换液一次
倍增时间：24-36 h
培养条件：气相：空气，温度：37℃
冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存
质量检测：细菌、真菌、支原体检测均为阴性
参考文献：
Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337.
Cruciger Q, et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976.
Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779.
Cailleau R, et al. Breast tumor cell lines from pleural effusions. J. Natl. Cancer Inst. 53: 661-674, 1974. PubMed: 4412247.
Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202.
Bates SE, et al. Expression of the transforming growth factor-alpha/epidermal growth factor receptor pathway in normal human breast epithelial cells. Endocrinology 126: 596-607, 1990. PubMed: 2294006.
Dickstein B, et al. Increased epidermal growth factor receptor in an estrogen-responsive, adriamycin-resistant MCF-7 cell line. J. Cell. Physiol. 157: 110-118, 1993. PubMed: 8408230.
Huguet EL, et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. PubMed: 8168088.
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