取自Sprague Dawley (SD) 大鼠脂肪,传代扩增至第二代冻存。本产品经生物安全检测,无细菌、真菌、霉菌、支原体和病毒污染,表达多种大鼠源间质干细胞特异性标记,具有良好的增殖和分化潜能,可分化为成骨细胞、脂肪细胞、软骨细胞等。
细胞名称:HyCyte™ SD大鼠脂肪间充质干细胞
细胞简称:SD ADSC
产品货号:ADRS-C106
种属来源:大鼠,Sprague Dawley品系
组织来源:脂肪
细胞形态:成纤维细胞样
生长特性:贴壁生长
培养基货号:ADRS-G102
传代比例:1:3-1:6,每2-3天换液一次
传代周期:48-72 h
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存
质量检测:细菌、真菌、支原体检测均为阴性
毒素检测:<10 EU/mL
参考文献:
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Song HY , Jeon ES,Jung JS, et al. Oncostatin M induces proliferation of human adipose tissue-derived mesenchymal stem cells[J]. Int J Biochem Cell Biol , 2005 ,37( 11 ):2357-2365.