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发货周期：
干冰冻存管运输3天（1x106cells/冻存管x1）
常温运温1-2周发货 (1x106 cells/T25培养瓶）
（推荐T25瓶，复苏发货1-2周）
该细胞来源于一名15岁的白人少年的肝癌组织。该细胞表达甲胎蛋白、白蛋白、α-2-巨球蛋白、α-1-抗胰蛋白酶、转铁蛋白、α-1-抗凝乳蛋白酶、结合珠蛋白、铜蓝蛋白、纤溶酶原、补体C4、C3激活物、纤维蛋白原、α-1酸性糖蛋白、α-2-HS-糖蛋白、β-脂蛋白、视黄醇结合蛋白；表达胰岛素受体和胰岛素样生长因子IGFⅡ的受体；该细胞具有3-羟基-3-甲酰辅酶A还原酶和肝甘油三酯脂肪酶的活性。目前尚未证明该细胞中有HBV基因组。
HepG2细胞是一种来源于人肝细胞癌（HCC）的细胞系，广泛应用于以下研究领域：药物代谢与毒性研究、肝癌生物学研究、代谢性疾病研究、外泌体与细胞间通信研究、生物活性物质筛选
无论您是研究药物代谢、肝癌机制，还是探索代谢性疾病的分子奥秘，HEPG2细胞都能为您提供稳定的实验支持。经过海星验证，HyCyte®人肝癌细胞(经非酒精性脂肪肝模型验证)HepG2在成脂诱导实验中表现出稳定的脂质积累和代谢基因调控能力，实验方法重复性高，适合用于脂质代谢相关研究，为您提供可靠的实验模型！
细胞名称：人肝癌细胞(经非酒精性脂肪肝模型验证)
细胞简称：HepG2
产品货号：TCH-C196-UD
种属来源：人
组织来源：肝
疾病特征：肝癌
细胞形态：上皮细胞样
生长特性：贴壁生长
培养体系：DMEM-H+10%FBS（胎牛血清）+1% Glutamax+1% Sodium Pyruvate+1%P/S
配套培养基货号：TCH-G196
传代比例：1:2-1:4，每2-3天换液一次
倍增时间：72-96 h
培养条件：气相：95%空气+5%CO2，温度：37℃
冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存
质量检测：细菌、真菌、支原体检测均为阴性
HepG2 已发表过的文章：
IF=39.3 Ma B, Ju A, Zhang S, et al. Albumosomes formed by cytoplasmic pre-folding albumin maintain mitochondrial homeostasis and inhibit nonalcoholic fatty liver disease[J]. Signal Transduction and Targeted Therapy, 2023, 8(1): 229.
IF=4.5 Guan H, Zhong M, Ma K, et al. The Comprehensive Role of High Mobility Group Box 1 (HMGB1) Protein in Different Tumors: A Pan-Cancer Analysis[J]. Journal of Inflammation Research, 2023: 617-637.
Chen Z, Wang Z, Liu W, et al. A Reproducible Hybrid Membrane for in Situ Analysis of Cell Secretions with a Wide Size Range[J].
参考文献：
"Knowles BB, Aden DP. Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983
Schardt C, et al. Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp. Cell Res. 204: 22-29, 1993. PubMed: 8380141
Cuthbert C, et al. Regulation of human apolipoprotein A-I gene expression by gramoxone. J. Biol. Chem. 272: 14954-14960, 1997. PubMed: 9169468
Deleersnyder V, et al. Formation of native hepatitis C virus glycoprotein complexes. J. Virol. 71: 697-704, 1997. PubMed: 8985401
Benn J, et al. Hepatitis B virus HBx protein induces transcription factor AP-1 by activation of extracellular signal-regulated and c-Jun N-terminal mitogen-activated protein kinases. J. Virol. 70: 4978-4985, 1996. PubMed: 8764004
Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260
Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128
Lewis W, et al. Fialuridine and its metabolites inhibit DNA polymerase gamma at sites of multiple adjacent analog incorporation, decrease mtDNA abundance, and cause mitochondrial structural defects in cultured hepatoblasts. Proc. Natl. Acad. Sci. USA 93: 3592-3597, 1996. PubMed: 8622980
Jang SI, et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. PubMed: 8798649
Roesler WJ, et al. The alpha-isoform of the CCAAT/enhancer-binding protein is required for mediating cAMP responsiveness of the phosphoenolpyruvate carboxykinase promoter in hepatoma cells. J. Biol. Chem. 271: 8068-8074, 1996. PubMed: 8626491
Lee JH, et al. The proximal promoter of the human transglutaminase 3 gene. J. Biol. Chem. 271: 4561-4568, 1996. PubMed: 8626812
Lieber A, et al. Recombinant adenoviruses with large deletions geneerated by cre-mediated excision exhibit different biological properties compared with first-generation vectors in vitro and in vivo. J. Virol. 70: 8944-8960, 1996. PubMed: 8971024
Dubuisson J, Rice CM. Hepatitis C virus glycoprotein folding: disulfide bond formation and association with calnexin. J. Virol. 70: 778-786, 1996. PubMed: 8551615"
收货当天如何处理细胞？
收到细胞后请务必仔细阅读产品资料，了解细胞相关信息，如贴壁特性 (贴壁/悬浮/半贴壁半悬浮)、细胞形态、培养体系、传代比例和换液频率等。
