取自Sprague Dawley (SD)大鼠骨髓,传代扩增至第二代冻存。本产品经生物安全检测,无细菌、真菌、霉菌、支原体和病毒污染,表达多种大鼠源间质干细胞特异性标记,具有良好的增殖和分化潜能,可分化为成骨细胞、脂肪细胞、软骨细胞等。
细胞名称:HyCyte™ (SD)大鼠骨髓间充质干细胞
细胞简称:SD MSC
产品货号:BMRS-C106
种属来源:大鼠,Sprague Dawley品系
组织来源:骨髓
细胞形态:成纤维细胞样
生长特性:贴壁生长
培养基货号:BMRS-G101
传代比例:1:3-1:6,每2-3天换液一次
传代周期:48-72 h
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存
质量检测:细菌、真菌、支原体检测均为阴性
毒素检测:<10 EU/mL
参考文献:
Delorme B.Chateauvicux S,Charbord P.The concept of mesenchymal stem cells[J门. Regen Med ,2006,1(4),497-509.
lzadpanah R,Joswing T, Tsien F,et al . Characterization of multipotent mesenchymal stem cells from the bone marrow ofrhesus macapues[J. Stem Cells Der ,2005,14(4);440-451.
Tondreau T,Lagneaux L,Dejeneffe M 、et al . Isolation of BM mesenchymal stem cells by plastic adhesion or negative selec-tion: phenotype , proliferation kinetics and differentiation po-tential[J]. Cytotherapy ,2004,6( 4):372-379.
Alborxi A,Mac KG,Lackin CA,et al . Endochondral and intramembranous fetal bone development ,osteoblastic cell prolif-cration and expression of alkaline phosphatasc,rn-t wist. andhistone H4[J门.Crani fac Genet Der Boil .1996,16(2);94-98.
Pittenger MF.Mackay AM ,Beck SC,et al . Multilineage potential of adult hurman mesenchymal stem cells[J]. Science ,1999,284(5411):143-147.