取自满月自然分娩的胎儿脐带血,传代扩增至第二代冻存。本产品经生物安全检测,无细菌、真菌、霉菌、支原体和病毒污染,表达多种人源间质干细胞特异性标记,具有良好的增殖和分化潜能,可分化为成骨细胞、脂肪细胞、软骨细胞等。
细胞名称:脐血来源人间充质干细胞
细胞简称:H UCB MSC
产品货号:UBHX-C106
种属来源:人
组织来源:脐血
细胞形态:成纤维细胞样
生长特性:贴壁生长
培养基货号:UBHX-G104
传代比例:1:3-1:6,每2-3天换液一次
传代周期:48-72 h
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存
质量检测:细菌、真菌、支原体检测均为阴性
毒素检测:<10 EU/mL
参考文献:
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Jang Y K,Jung D H,Jung M H, et al. Mesenchymal stem cellsfeeder layer from human umbilical cord blood for ex vivo expandedgrowth and proliferation of hematopoietic progenitor cells. Annalsof Hematology, 2006,85(4): 212~225
Yang S E,Ha C w,Jung M, et al. Mesenchymal stem/progenitor cells developed in cultures from UC blood.Cytotherapy,2004,6(5):476~486