取自18-45岁成人脂肪,传代扩增至第二代冻存。本产品经生物安全检测,无细菌、真菌、霉菌、支原体和病毒污染,表达多种人源间质干细胞特异性标记,具有良好的增殖和分化潜能,可分化为成骨细胞、脂肪细胞、软骨细胞等。
细胞名称:HyCyte™成人脂肪间充质干细胞
细胞简称:H ADSC
产品货号:ADHX-C106
种属来源:人
组织来源:脂肪
细胞形态:成纤维细胞样
生长特性:贴壁生长
培养基货号:ADHX-G102
传代比例:1:3-1:6,每2-3天换液一次
传代周期:48-72 h
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存
质量检测:细菌、真菌、支原体检测均为阴性
毒素检测:<10 EU/mL
参考文献:
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Bassi G, Pacelli L, Carusone R, et ai. Adipose-derived stromal cells(ASCs)[J]. Transfus Apher Sci, 2012,47(2):193
DelaRosa O, Sanchez-Correa B, Morgado S, et al. Human adipose derived stem cells impair natural killer cell function and exhibitlow susceptibility to natural killer mediated lysis[J]. Stem Cells Dev,2012,21(8):1333
Estes B T,Diekman B O,Gimble J M, et al. Isolation of adipose derived stem cells and their induction to a chondrogenic phenotype[J]. Nat Protoc,2010,5(7):1294
Bassi G,Pacelli L, Carusone R, et al. Adipose derived stromal cells (ASCs)[J].Transfus Apher Sci,2012,47(2): 193