HyCyte® | 海星生物

发货周期：

干冰冻存管运输3天（1x10⁶cells/冻存管x2）

常温运温1-2周发货 (1x10⁶ cells/T25培养瓶）

原价：1000元   套装价特惠价：890元

该细胞系是1972年由GiardDJ通过肺癌组织移植培养建系的，源自一位58岁的白人男性。A549能通过胞苷二磷脂酰胆碱途径合成富含不饱和脂肪酸的卵磷脂；角蛋白阳性。

细胞名称：人非小细胞肺癌细胞(STR鉴定正确)

细胞简称：A549

产品货号：TCH-C116

种属来源：人

组织来源：肺

疾病特征：肺癌

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系：D/F12+10%FBS+1%P/S

配套培养基货号：TCH-G116

传代比例：1:3-1:6，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO₂，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献："Letko M, et al. Functional assessment of cell entry and receptor usage for SARS-CoV-2 and other lineage B betacoronaviruses. Nat Microbiol 5(4): 562-569, 2020. PubMed: 32094589

Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758

Mayr GA, Freimuth P. A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells. J. Virol. 71: 412-418, 1997. PubMed: 8985365

Goodrum FD, Ornelles DA. The early region 1B 55-kilodalton oncoprotein of adenovirus relieves growth restrictions imposed on viral replication by the cell cycle. J. Virol. 71: 548-561, 1997. PubMed: 8985383

St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830

Horikami SM, et al. The Sendai virus V protein interacts with the NP protein to regulate viral genome RNA replication. Virology 222: 383-390, 1996. PubMed: 8806522

Huang S, et al. Adenovirus interaction with distinct integrins mediates separate events in cell entry and gene delivery to hematopoietic cells. J. Virol. 70: 4502-4508, 1996. PubMed: 8676475"

A549 已发表过的文章：

IF=8.4 Wang L L, Xu Q, Xie Y Z, et al. A triple-targeting fluorescent probe reveals the glutathione and viscosity crosstalk in mitochondria, endoplasmic reticulum, and nucleoli in cells during ferroptosis[J]. Sensors and Actuators B: Chemical, 2024, 399: 134872.

IF=4.9 Zhang Y, Wang Y, Chen Y, et al. PET Imaging of Peptide Probe Al [18F] F-NOTA-PCP1 for Monitoring the Engagement of PD-L1 Antibodies in Tumors[J]. Molecular Pharmaceutics, 2024.

IF=3.4 Li J H, Wan H X, Wu L H, et al. Calcitonin gene‑related peptide alleviates hyperoxia‑induced human alveolar cell injury via the CGRPR/TRPV1/Ca2+ axis[J]. Molecular Medicine Reports, 2024, 30(1): 1-14.

IF=2.9 Li P, Ma G, Cui Z, et al. FOXM1 and CENPF are associated with a poor prognosis through promoting proliferation and migration in lung adenocarcinoma[J]. Oncology Letters, 2023, 26(6): 1-13.

收货当天如何处理细胞？

收到细胞后请务必仔细阅读产品资料，了解细胞相关信息，如贴壁特性 (贴壁/悬浮/半贴壁半悬浮)、细胞形态、培养体系、传代比例和换液频率等。