发货周期:
干冰冻存管运输3天(1x106cells/冻存管x2)
常温运温1-2周发货 (1x106 cells/T25培养瓶)
原价:1500元 套装价特惠价:890元
这株细胞来源于一个淋巴结转移。患者接受了初期放疗。细胞均一性的部分缺失p53蛋白,并缺少p53蛋白表达。细胞可以合成0.1pmol/毫克蛋白的NMB蛋白,而不合成促胃液释放肽(GRP)。
细胞名称:人非小细胞肺癌细胞(STR鉴定正确)
细胞简称:NCI-H1299
产品货号:TCH-C269
种属来源:人
组织来源:肺
疾病特征:非小细胞肺癌
细胞形态:上皮样/多角形
生长特性:贴壁生长
培养体系:RPMI-1640+10%FBS+1%P/S
配套培养基货号:TCH-G269
传代比例:1:3-1:6,每2-3天换液一次
传代周期:48-72 h
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存
质量检测:细菌、真菌、支原体检测均为阴性
参考文献:
Giaccone G, et al. Neuromedin B is present in lung cancer cell lines. Cancer Res. 52: 2732s-2736s, 1992. PubMed: 1563005
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
Lin DL, Chang C. p53 is a mediator for radiation-repressed human TR2 orphan receptor expression in MCF-7 cells, a new pathway from tumor suppressor to member of the steroid receptor superfamily. J. Biol. Chem. 271: 14649-14652, 1996. PubMed: 8663350
This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lymph node metastasis from a patient who had received prior radiation therapy.
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