发货周期:
干冰冻存管运输3天(1x106cells/冻存管x2)
常温运温1-2周发货 (1x106 cells/T25培养瓶)
原价:1500元 套装价特惠价:890元
注意:
1)该细胞推荐使用Leibovitz's L-15培养基进行培养,Leibovitz's L-15不可以通入二氧化碳,会产生细胞毒性;
2)如您没有无二氧化碳的培养箱,可使用DMEM替代Leibovitz's L-15,使用DMEM培养基时即可正常通入5%二氧化碳;
3)配套专用培养基默认Leibovitz's L-15配置,如需DMEM配方,请联系销售下单备注更改为人乳腺癌细胞(DMEM-H培养) 货号TCH-C453。
MDA-MB-231是从一名51岁的白人女性乳腺癌患者的胸水中分离建立的。该细胞表达表皮生长因子EGF受体、TGF-α受体和WNT7B癌基因。
细胞名称:人乳腺癌细胞(L-15培养)(STR鉴定正确)
细胞简称:MDA-MB-231
产品货号:TCH-C248
种属来源:人
组织来源:乳腺
疾病特征:乳腺癌
细胞形态:上皮细胞样
生长特性:贴壁生长
培养体系:L-15+10%FBS+1%P/S
配套培养基货号:TCH-G248
传代比例:1:3-1:6,每2-3天换液一次
传代周期:48-72 h
培养条件:气相:空气,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存
质量检测:细菌、真菌、支原体检测均为阴性
参考文献:
Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337
Cruciger Q, et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976.
Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779
Cailleau R, et al. Breast tumor cell lines from pleural effusions. J. Natl. Cancer Inst. 53: 661-674, 1974. PubMed: 4412247
Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202
Bates SE, et al. Expression of the transforming growth factor-alpha/epidermal growth factor receptor pathway in normal human breast epithelial cells. Endocrinology 126: 596-607, 1990. PubMed: 2294006
Dickstein B, et al. Increased epidermal growth factor receptor in an estrogen-responsive, adriamycin-resistant MCF-7 cell line. J. Cell. Physiol. 157: 110-118, 1993. PubMed: 8408230
Huguet EL, et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. PubMed: 8168088
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