HyCyte® | 海星生物

产品名称：HGC-27细胞hPIK3R1基因过表达稳转株

产品货号：CGSC-M25331

基因名称：hPIK3R1

基因ID：   5295

转导方法：转座子

转录本：[NM_181523.3]/3xFLAG

抗性基因：puro

荧光基因：EGFP

验证方式：qPCR

克隆类型：Poolcells

种属来源：人

组织来源：胃

疾病特征：胃癌

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系：RPMI-1640+10%FBS+1%P/S

配套培养基货号：TCH-G202

传代比例：1:3-1:6，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO₂，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

"Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Cancer Res. 79:1263-1273(2019)

Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

A landscape of pharmacogenomic interactions in cancer.

Cell 166:740-754(2016)

Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.

A comprehensive transcriptional portrait of human cancer cell lines.

Nat. Biotechnol. 33:306-312(2015)

Liu J., McCleland M., Stawiski E.W., Gnad F., Mayba O., Haverty P.M., Durinck S., Chen Y.-J., Klijn C., Jhunjhunwala S., Lawrence M., Liu H., Wan Y., Chopra V., Yaylaoglu M.B., Yuan W., Ha C., Gilbert H.N., Reeder J., Pau G., Stinson J., Stern H.M., Manning G., Wu T.D., Neve R.M., de Sauvage F.J., Modrusan Z., Seshagiri S., Firestein R., Zhang Z.

Integrated exome and transcriptome sequencing reveals ZAK isoform usage in gastric cancer.

Nat. Commun. 5:3830-3830(2014)

Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Cancer Res. 70:2158-2164(2010)"

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