890.00
人慢性髓原白血病细胞(K-562)
品质保证
干冰运输另加200元
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STR检测
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类型
细胞系
产品货号
TCH-C226
品牌
HyCyte®
价格
890.00
细胞别名
K562; K.562; K 562; KO; GM05372; GM05372E
库存量
100
规格
1X10^6
用途
仅供科研使用

发货周期:

干冰冻存管运输3天(1x106cells/冻存管x2)

常温运温1-2周发货 (1x106 cells/T25培养瓶)


原价:1500元   套装价特惠价:890元


该细胞适合做转染宿主,来自于一位53岁女性慢性粒细胞白血病人的胸腔积液。

细胞名称:人慢性髓原白血病细胞(STR鉴定正确)

细胞简称:K-562

产品货号:TCH-C226

种属来源:人

组织来源:骨髓

疾病特征:慢性骨髓性白血病

细胞形态:淋巴母细胞样

生长特性:悬浮生长

培养体系:IMDM+10%FBS+1%P/S

配套培养基货号:TCH-G226

传代比例:1:3-1:6,每2-3天换液一次

传代周期:24-48 h

培养条件:气相:95%空气+5%CO2,温度:37℃

冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存

质量检测:细菌、真菌、支原体检测均为阴性

参考文献:

"Koeffler HP, Golde DW. Human myeloid leukemia cell lines: a review. Blood 56: 344-350, 1980. PubMed: 6996765

Ortaldo JR, et al. Specificity of natural cytotoxic reactivity of normal human lymphocytes against a myeloid leukemia cell line. J. Natl. Cancer Inst. 59: 77-82, 1977. PubMed: 69036

Lozzio CB, Lozzio BB. Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. Blood 45: 321-334, 1975. PubMed: 163658

Lozzio BB, Lozzio CB. Properties and usefulness of the original K-562 human myelogenous leukemia cell line. Leuk. Res. 3: 363-370, 1979. PubMed: 95026

Andersson LC, et al. K562--a human erythroleukemic cell line. Int. J. Cancer 23: 143-147, 1979. PubMed: 367973

Lozzio BB, et al. A multipotential leukemia cell line (K-562) of human origin. Proc. Soc. Exp. Biol. Med. 166: 546-550, 1981. PubMed: 7194480

Dimery IW, et al. Variation amongst K562 cell cultures. Exp. Hematol. 11: 601-610, 1983. PubMed: 6576909

Chan YJ, et al. Two distinct upstream regulatory domains containing multicopy cellular transcription factor binding sites provide basal repression and inducible enhancer characteristics to the immediate-early IES (US3) promoter from human cytomegalovirus. J. Virol. 70: 5312-5328, 1996. PubMed: 8764042

Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128

Gan W, Rhoads RE. Internal initiation of translation directed by the 5'-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation. J. Biol. Chem. 271: 623-626, 1996. PubMed: 8557663

Tiffany HL, et al. Enhanced expression of the eosinophil-derived neurotoin ribonuclease (RNS2) gene requires interaction between the promoter and intron. J. Biol. Chem. 271: 12387-12393, 1996. PubMed: 8647842"


收货当天如何处理细胞?

收到细胞后请务必仔细阅读产品资料,了解细胞相关信息,如贴壁特性 (贴壁/悬浮/半贴壁半悬浮)、细胞形态、培养体系、传代比例和换液频率等。

点击可查看下载《细胞收货处理方法说明》.jpg


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