HyCyte® | 海星生物

发货周期：

干冰冻存管运输3天（1x10⁶cells/冻存管x2）

常温运温1-2周发货 (1x10⁶ cells/T25培养瓶）

原价：1500元   套装价特惠价：890元

该细胞从一名1岁的患有急性单核细胞性白血病的男孩的外周血中分离建立。该细胞可以吞噬乳胶颗粒和激活的红细胞，细胞膜和胞浆内均没有免疫球蛋白，表达C3R和FcR；可受佛波酯TPA诱导向单核系方向分化；可作为转染宿主。

细胞名称：人单核细胞白血病（STR鉴定正确）

细胞简称：THP-1

产品货号：TCH-C361

种属来源：人

组织来源：外周血

疾病特征：急性单核细胞白血病

细胞形态：单核细胞样

生长特性：悬浮生长

培养体系：RPMI-1640+10%FBS+0.05 mM β-mercaptoethanol+1%P/S

配套培养基货号：TCH-G361

传代比例：1:3-1:6，每2-3天换液一次

传代周期：24-48 h

培养条件：气相：95%空气+5%CO₂，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

"Tsuchiya S, et al. Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester. Cancer Res. 42: 1530-1536, 1982. PubMed: 6949641

Skubitz KM, et al. Human granulocyte surface molecules identified by murine monoclonal antibodies. J. Immunol. 131: 1882-1888, 1983. PubMed: 6619543

Cuthbert JA, Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed: 9270019

Huang S, et al. Adenovirus interaction with distinct integrins mediates separate events in cell entry and gene delivery to hematopoietic cells. J. Virol. 70: 4502-4508, 1996. PubMed: 8676475

Clark RA, et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997. PubMed: 9151679

Hambleton J, et al. Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages. Proc. Natl. Acad. Sci. USA 93: 2774-2778, 1996. PubMed: 8610116

Hsu HY, et al. Inhibition of macrophage scavenger receptor activity by tumor necrosis factor-alpha is transcriptionally and post-transcriptionally regulated. J. Biol. Chem. 271: 7767-7773, 1996. PubMed: 8631819

Lucas M, Mazzone T. Cell surface proteoglycans modulate net synthesis and secretion of macrophage apolipoprotein E. J. Biol. Chem. 271: 13454-13460, 1996. PubMed: 8662812

Sando GN, et al. Induction of ceramide glucosyltransferase activity in cultured human keratinocytes. J. Biol. Chem. 271: 22044-22051, 1996. PubMed: 8703011

Ollivier V, et al. Elevated cyclic AMP inhibits NF-kappaB-mediated transcription in human monocytic cells and endothelial cells. J. Biol. Chem. 271: 20828-20835, 1996. PubMed: 8702838

Tsuchiya S, et al. Establishment and characterization of a human acute monocytic leukemia cell line (THP-1). Int. J. Cancer 26: 171-176, 1980. PubMed: 6970727"

THP-1 已发表过的文章：

IF=8.2 Jiang F, Wu G, Yang H, et al. Diethylaminoethyl-dextran and monocyte cell membrane coated 1, 8-cineole delivery system for intracellular delivery and synergistic treatment of atherosclerosis[J]. International Journal of Biological Macromolecules, 2023, 253: 127365.

IF=7.9 Che N, Li M, Liu X, et al. Macelignan prevents colorectal cancer metastasis by inhibiting M2 macrophage polarization[J]. Phytomedicine, 2024, 122: 155144.

IF=5.8 Qi D, Lu Y, Qu H, et al. Independent prognostic value of CLDN6 in bladder cancer based on M2 macrophages related signature[J]. Iscience, 2024.

IF=4.7 Wang Y, Mao J, Wang Y, et al. Multifunctional Exosomes Derived from M2 Macrophages with Enhanced Odontogenesis, Neurogenesis and Angiogenesis for Regenerative Endodontic Therapy: An In Vitro and In Vivo Investigation[J]. Biomedicines, 2024, 12(2): 441.

IF=2.7 Du Y, Liu X, Xiao C, et al. TIPE2 regulates periodontal inflammation by inhibiting NF-κB p65 phosphorylation[J]. Journal of Applied Oral Science, 2023, 31: e20230162.

IF=2.5 Huo R, Zhao R, Li Z, et al. APOE expression in papillary thyroid carcinoma: Influencing tumor progression and macrophage polarization[J]. Immunobiology, 2024: 152821.

THP-1细胞常见问题及解决方法：

1. 培养基里一定要加β-巯基乙醇（ β-mercaptoethanol）吗？

β-巯基乙醇是一种常用的培养补充剂，有抗氧化的作用，可减少氧化应激对THP-1细胞的影响。当细胞密度过大但需要维持时，可适当增加巯基乙醇的比例（不超过标准量的1.5倍）。

2. 悬浮细胞在镜下观察时，难以聚焦或估算密度怎么办？

悬浮细胞会随着液体流动，不容易聚焦，静置5-10分钟使细胞沉底，之后再轻轻放上显微镜观察，将有助于聚焦和估算细胞密度。

3.THP-1细胞在培养过程中细胞发生聚团怎么办？

少量细胞聚团，呈葡萄串状，属于正常现象，特别是细胞密度较低时，易出现这种情况。更换血清品牌或增大血清比例（不超过20%）有助于解决聚团问题。不建议将聚团的细胞吹散，等待密度高时，会自己分散开。

THP-1细胞在密度较高时不聚团

4. THP-1细胞在培养过程中细胞发生贴壁怎么办？

少量细胞贴壁属于正常情况，将细胞悬液转移至新瓶中即可。当贴壁细胞的比例高于20%，说明细胞生长环境有异常，需排查培养箱设置、培养基成分，以及培养器皿是否异常。

收货当天如何处理细胞？

收到细胞后请务必仔细阅读产品资料，了解细胞相关信息，如贴壁特性 (贴壁/悬浮/半贴壁半悬浮)、细胞形态、培养体系、传代比例和换液频率等。