HyCyte® | 海星生物

发货周期：

干冰冻存管运输3天（1x10⁶cells/冻存管x2）

常温运输1-2周发货 (1x10⁶ cells/T25培养瓶）

（推荐T25瓶，复苏发货1-2周）

原价：1500元   套装价特惠价：890元

此细胞株源自BALB/c小鼠由Abelson鼠科白血病病毒诱导的肿瘤。可产生溶菌酶；sIg-,Ia-，Thy-1.2-。为检测到病毒颗粒的分泌，XC斑点形成试验阴性。该细胞可以胞饮中性红并吞噬乳胶颗粒与酵母聚糖；可以经抗体依赖分裂绵羊红细胞与肿瘤靶细胞；LPS或PPD处理2天可诱导分裂红细胞，但对肿瘤靶细胞无作用。

细胞名称：小鼠单核巨噬细胞白血病细胞

细胞简称：RAW 264.7

产品货号：TCM-C766

种属来源：小鼠

组织来源：单核细胞；巨噬细胞

疾病特征：Abelson鼠科白血病病毒诱导的肿瘤

细胞形态：不规则圆形

生长特性：贴壁生长

培养体系：DMEM-H+10%FBS+1%Glutamax+1% Sodium Pyruvate+1%P/S

配套培养基货号：TCM-G766

传代比例：1:3-1:6，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO₂，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

RAW264.7 已发表过的文章：

IF=15.8 Yang X, Fan Y, Liang J, et al. Polyaptamer-Driven Crystallization of Alendronate for Synergistic Osteoporosis Treatment through Osteoclastic Inhibition and Osteogenic Promotion. ACS Nano. 2024;18(33):22431-22443.

IF=8.2 Li F, Liu T, Liu X, et al. Ganoderma lucidum polysaccharide hydrogel accelerates diabetic wound healing by regulating macrophage polarization[J]. International Journal of Biological Macromolecules, 2024, 260: 129682.

IF=7.9 Che N, Li M, Liu X, et al. Macelignan prevents colorectal cancer metastasis by inhibiting M2 macrophage polarization[J]. Phytomedicine, 2024, 122: 155144.

IF=6.1 Geng R, Zhao Y, Xu W, et al. SIRPB1 regulates inflammatory factor expression in the glioma microenvironment via SYK: functional and bioinformatics insights[J]. Journal of Translational Medicine, 2024, 22(1): 338.

IF=5.6 He S, Yan J, Chen L, et al. Structure and in vitro antioxidant and immunomodulatory activity of a glucan from the leaves of Cyclocarya paliurus[J]. Journal of Functional Foods, 2024, 113: 106016.

IF=5.2 Chen J, Lv Y, Shang W, et al. Loaded delta-hemolysin shapes the properties of Staphylococcus aureus membrane vesicles[J]. Frontiers in Microbiology, 2023, 14: 1254367.

IF=4.8 Chen T, Zhou X, Zhu M, et al. Phytochemical determination and mechanistic investigation of Polygala tenuifolia root (Yuanzhi) extract for bronchitis: UPLC-MS/MS analysis, network pharmacology and in vitro/in vivo evaluation[J]. Journal of Ethnopharmacology, 2024: 118418.

IF=4.6 Yang Y, Tang X, Yao T, et al. Metformin protects ovarian granulosa cells in chemotherapy-induced premature ovarian failure mice through AMPK/PPAR-γ/SIRT1 pathway[J]. Scientific Reports, 2024, 14(1): 1447.

IF=4.1 Chen G, Shen L, Hu H, et al. Sulforaphane Inhibits Oxidative Stress and May Exert Anti-Pyroptotic Effects by Modulating NRF2/NLRP3 Signaling Pathway in Mycobacterium tuberculosis-Infected Macrophages[J]. Microorganisms, 2024, 12(6): 1191.

Li W, Peng Y, Liu J, et al. Discovery and Synthesis of Novel Glyrrhizin-Analogs Containing Furanoylpiperazine and the Activity Against Myocardial Injury in Sepsis[J]. Available at SSRN 4821740.

参考文献：

"Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031

Raschke WC, et al. Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198

Denlinger LC, et al. Regulation of inducible nitric oxide synthase expression by macrophage purinoreceptors and calcium. J. Biol. Chem. 271: 337-342, 1996. PubMed: 8550583

Hambleton J, et al. Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages. Proc. Natl. Acad. Sci. USA 93: 2774-2778, 1996. PubMed: 8610116

Taylor GA, et al. Identification of anovel GTPase, the inducibly expresed GTPase, that accumulates in response to interferon gamma. J. Biol. Chem. 271: 20399-20405, 1996. PubMed: 8702776

Li YM, et al. Molecular identity and cellular distribution of advanced glycation endproduct receptors: relationship of p60 to OST-48 and p90 to 80K-H membrane proteins. Proc. Natl. Acad. Sci. USA 93: 11047-11052, 1996. PubMed: 8855306

Panneerselvam K, Freeze HH. Mannose enters mammalian cells using a specific transporter that is insensitive to glucose. J. Biol. Chem. 271: 9417-9421, 1996. PubMed: 8621609

Lokuta MA, et al. Mechanisms of murine RANTES chemokine gene induction by newcatle disease virus. J. Biol. Chem. 271: 13731-13738, 1996. PubMed: 8662857

Taylor MF, et al. In vitro efficacy of morpholino-modified antisense oligomers directed against tumor necrosis factor-alpha mRNA. J. Biol. Chem. 271: 17445-17452, 1996. PubMed: 8663413

Standard Practice for Testing for Biological Responses to Particles in Vitro. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 1903-98R03.

Hartley JW, et al. Expression of infectious murine leukemia viruses by RAW264.7 cells, a potential complication for studies with a widely used mouse macrophage cell line. Retrovirology. 4: 5:1, 2008. PubMed 18177500."

收货当天如何处理细胞？

收到细胞后请务必仔细阅读产品资料，了解细胞相关信息，如贴壁特性 (贴壁/悬浮/半贴壁半悬浮)、细胞形态、培养体系、传代比例和换液频率等。