发货周期:
干冰冻存管运输3天(1x106cells/冻存管x2)
常温运温1-2周发货 (1x106 cells/T25培养瓶)
原价:1400元 套装价特惠价:890元
Hep-3b细胞建系于1979年,源自一位患有肝癌的7岁黑人男童。该细胞产生甲胎蛋白(alpha-fetoprotein)、乙肝表面抗原(BHsAg)、白蛋白、巨球蛋白、α-抗胰蛋白酶(alphal-antitrypsin)、转铁蛋白、补体(C3)、C3活性载体,纤维原等,具有广泛的研究价值。
细胞名称:人肝癌细胞(STR鉴定正确)
细胞简称:Hep3B2.1-7
产品货号:TCH-C195
种属来源:人
组织来源:肝脏
疾病特征:肝癌
细胞形态:上皮细胞样
生长特性:贴壁生长
培养体系:MEM+10%FBS+1%Glutamax+1%NEAA+1% Sodium Pyruvate+1%P/S
配套培养基货号:TCH-G195
传代比例:1:2-1:3,每2-3天换液一次
传代周期:72-96 h
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存
质量检测:细菌、真菌、支原体检测均为阴性
参考文献:
Knowles BB, et al. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499, 1980. PubMed: 6248960
Knowles BB, Aden DP. Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983
Aden DP, et al. Controlled synthesis of HBsAg in a differentiated human liver carcinoma- derived cell line. Nature 282: 615-616, 1979. PubMed: 233137
Darlington GJ, et al. Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354, 1987. PubMed: 3034851
Knowles BB, Aden DP. Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983
Hep3B2.1-7 已发表过的文章:
IF=3.8 Yang K, Ma Y, Chen W, et al. CCDC58 is a potential biomarker for diagnosis, prognosis, immunity, and genomic heterogeneity in pan-cancer[J]. Scientific Reports, 2024, 14(1): 8575.
Yu C, Ma Y. Stigmasterol inhibits the progression of hepatocellular carcinoma by modulating the TLR4/MyD88/NF-κB signaling pathway[J]. 2024.
收货当天如何处理细胞?
收到细胞后请务必仔细阅读产品资料,了解细胞相关信息,如贴壁特性 (贴壁/悬浮/半贴壁半悬浮)、细胞形态、培养体系、传代比例和换液频率等。