HyCyte® | 海星生物

产品名称：EL4细胞mTmem176b基因敲除株

产品简称：EL4 KO-mTmem176b

产品货号：CGKO-M2284

修饰基因：mTmem176b

基因ID：65963

修饰类型：敲除

转导方法：蛋白电转

抗性基因：—

克隆类型：纯合子

细胞鉴定：PCR，测序

种属来源：小鼠

组织来源：T淋巴细胞

疾病特征：淋巴瘤

细胞形态：淋巴母细胞样

生长特性：悬浮生长

培养体系：DMEM-H＋10% FBS＋1% P/S

配套培养基货号：TCM-G727

传代比例：3×10^5-5×10^5cells/mL，每2-3天换液一次

传代周期：24-48 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304 Old LJ, et al. The G (Gross) leukemia antigen. Cancer Res. 25: 813-819, 1965. PubMed: 4284252 Gorer PA. Studies in antibody response of mice to tumour inoculation. Br. J. Cancer 4: 372-379, 1950. PubMed: 14801344 Herberman RB. Serological analysis of cell surface antigens of tumors induced by murine leukemia virus. J. Natl. Cancer Inst. 48: 265-271, 1972. PubMed: 4119883 Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714 Shevach EM, et al. Immunoglobulin and theta-bearing murine leukemias and lymphomas. J. Immunol. 108: 1146-1151, 1972. PubMed: 4112916 Aparicio CL, et al. Correction for label leakage in fluorimetric assays of cell adhesion. BioTechniques 23: 1056-1060, 1997. PubMed: 9421636 Cuthbert JA, Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed: 9270019

收货当天如何处理细胞？

收到细胞后请务必仔细阅读产品资料，了解细胞相关信息，如贴壁特性 (贴壁/悬浮/半贴壁半悬浮)、细胞形态、培养体系、传代比例和换液频率等。