产品名称:B16-F10细胞mTmem176b基因敲除株
产品简称:B16-F10 KO-mTmem176b
产品货号:CGKO-M2280
修饰基因:mTmem176b
基因ID:65963
修饰类型:敲除
转导方法:蛋白电转
抗性基因:—
克隆类型:纯合子
细胞鉴定:PCR,测序
种属来源:小鼠
组织来源:皮肤
疾病特征:黑色素瘤
细胞形态:上皮细胞样混有梭形细胞
生长特性:贴壁生长
培养体系:DMEM-H+10%FBS+1%P/S
配套培养基货号:TCM-G713
传代比例:1:3-1:6,每2-3天换液一次
传代周期:48-72 h
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存
质量检测:细菌、真菌、支原体检测均为阴性
参考文献:
Fidler IJ. Biological behavior of malignant melanoma cells correlated to their survival in vivo. Cancer Res. 35: 218-224, 1975. PubMed: 1109790
Fidler IJ, et al. Tumoricidal properties of mouse macrophages activated with mediators from rat lymphocytes stimulated with concanavalin A. Cancer Res. 36: 3608-3615, 1976. PubMed: 953987
Fidler IJ, Bucana C. Mechanism of tumor cell resistance to lysis by syngeneic lymphocytes. Cancer Res. 37: 3945-3956, 1977. PubMed: 908034
Fidler IJ, Kripke ML. Metastasis results from preexisting variant cells within a malignant tumor. Science 197: 893-895, 1977. PubMed: 887927
Briles EB, Kornfeld S. Isolation and metastatic properties of detachment variants of B16 melanoma cells. J. Natl. Cancer Inst. 60: 1217-1222, 1978. PubMed: 418183
Fidler IJ. Selection of successive tumour lines for metastasis. Nat. New Biol. 242: 148-149, 1973. PubMed: 4512654
Li M, et al. Loss of intracisternal A-type retroviral particles in BL6 melanoma cells transfected with MHC class I genes. J.Gen. Virol. 77: 2757-2765, 1996. PubMed: 8922469
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