HyCyte® | 海星生物

细胞名称：CHO-S细胞Glul基因敲除株

细胞简称：CHO-S KO-Glul

产品货号：CGKO-M2186

修饰基因：Glul(ID: 100764163)

修饰类型：敲除

转导方法：蛋白电转

抗性基因：—

克隆类型：纯合子

细胞鉴定：PCR，测序

种属来源：仓鼠

组织来源：卵巢

疾病特征：卵巢癌

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系：CHO-S专用培养基+1%Glutamax

传代比例：1:3-1:6，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存 (推荐海星一步冻存液GUCP-R201)

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

Puck TT, et al. Genetics of somatic mammalian cells III. Long-term cultivation of euploid cells from human and animal subjects. J. Exp. Med. 108: 945-956, 1958. PubMed: 13598821

Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc. Natl. Acad. Sci. USA 53: 288-293, 1965. PubMed: 14294058

Kao FT, Puck TT. Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells. Proc. Natl. Acad. Sci. USA 60: 1275-1281, 1968. PubMed: 5244736

Kao FT, Puck TT. Genetics of somatic mammalian cells. IV. Properties of Chinese hamster cell mutants with respect to the requirement for proline. Genetics 55: 513-524, 1967. PubMed: 6068403

Kao FT, et al. Complementation analysis on virus-fused Chinese hamster cells with nutritional markers. Science 164: 312-314, 1969. PubMed: 5813351

Kao F, et al. Genetics of somatic mammalian cells. X. Complementation analysis of glycine-requiring mutants. Proc. Natl. Acad. Sci. USA 64: 1284-1291, 1969. PubMed: 5271752