细胞名称:SiHa细胞human NAT10基因敲除株
细胞简称:SiHa KO-hNAT10
产品货号:CGKO-M2134
修饰基因:hNAT10
修饰类型:敲除
转导方法:慢病毒转导
抗性基因:Puro
克隆类型:纯合子
细胞鉴定:PCR,测序,WB
种属来源:人
组织来源:宫颈
疾病特征:宫颈鳞癌
细胞形态:上皮细胞样
生长特性:贴壁生长
培养体系:DMEM-H+10%FBS+1%P/S
配套培养基货号:TCH-G326
传代比例:1:3-1:6,每2-3天换液一次
传代周期:48-72 h
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存
质量检测:细菌、真菌、支原体检测均为阴性
参考文献:
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Pater MM, Pater A. Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145: 313-318, 1985. PubMed: 2992153
Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217
Friedl F, et al. Studies on a new human cell line (SiHa) derived from carcinoma of uterus. I. Its establishment and morphology. Proc. Soc. Exp. Biol. Med. 135: 543-545, 1970. PubMed: 5529598
Scheffner M, et al. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991. PubMed: 1648218
Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105
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