HyCyte® | 海星生物

产品名称：U-937细胞hPTPRA基因敲除株

产品简称：U-937 KO-hPTPRA

产品货号：CGKO-M2708

修饰基因：hPTPRA

基因ID：5786

修饰类型：敲除

转导方法：蛋白法

抗性基因：—

克隆类型：杂合子

细胞鉴定：PCR，测序

种属来源：人

组织来源：胸膜/胸腔积液

疾病特征：淋巴瘤细胞

细胞形态：单核细胞

生长特性：悬浮生长

培养体系：RPMI-1640+10%FBS+1%P/S

配套培养基货号：TCH-G368

传代比例：1:3-1:6，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

Ralph P, et al. Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. J. Exp. Med. 143: 1528-1533, 1976. PubMed: 1083890

. Gene expression during normal and malignant differentiation. London: Academic Press; 1985.

. International symposium on new trends in human immunology and cancer immunotherapy. Paris: Doin Editeurs; 1980.

Koren HS, et al. In vitro activation of a human macrophage-like cell line. Nature 279: 328-331, 1979. PubMed: 450085

Gidlund M, et al. Natural killer cells kill tumour cells at a given stage of differentiation. Nature 292: 848-850, 1981. PubMed: 7266653

Olsson I, et al. Induction of differentiation of the human histiocytic lymphoma cell line U-937 by 1 alpha,25-dihydroxycholecalciferol. Cancer Res. 43: 5862-5867, 1983. PubMed: 6315218

Morimoto H, et al. Overcoming tumor necrosis factor and drug resistance of human tumor cell lines by combination treatment with anti-Fas antibody and drugs or toxins. Cancer Res. 53: 2591-2596, 1993. PubMed: 7684321

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