产品名称:KYSE-150细胞hDYRK2基因敲除株
产品简称:KYSE-150 KO-hDYRK2
产品货号:CGKO-M2614
修饰基因:hDYRK2
基因ID:8445
修饰类型:敲除
转导方法:蛋白法
抗性基因:—
克隆类型:纯合子
细胞鉴定:PCR,测序
种属来源:人
组织来源:食管
疾病特征:食管鳞癌
细胞形态:上皮细胞样
生长特性:贴壁生长
培养体系:RPMI-1640 +10% FBS+1% P/S
配套培养基货号:TCH-G236
传代比例:1:2-1:4,每2-3天换液一次
传代周期:18-24 h
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存
推荐海星HyCyte®一步冻存液(即用型、无血清、无需程序降温),货号:GUCP-R201
质量检测:细菌、真菌、支原体检测均为阴性
参考文献:
Kanda, Y., Nishiyama, Y., Shimada, Y., Imamura, M., Nomura, H., Hiai, H., Fukumoto, M. (1994). Analysis of gene amplification and overexpression in human esophageal-carcinoma cell lines. International journal of cancer.Journal international du cancer 58 ( 2 ): 291-297 . Http://www.ncbi.nlm.nih.gov/pubmed/7913084 Shimada, Y., Imamura, M., Wagata, T., Yamaguchi, N., Tobe, T. (1992). Characterization of 21 newly established esophageal cancer cell lines. Cancer 69 ( 2 ): 277-284 . Http://www.ncbi.nlm.nih.gov/pubmed/1728357 Shimada, Y., Imamura, M. (1993). Prognostic significance of cell culture in carcinoma of the oesophagus. The British journal of surgery 80 ( 5 ): 605-607 . Http://www.ncbi.nlm.nih.gov/pubmed/8518899 Tanaka, H., Shibagaki, I., Shimada, Y., Wagata, T., Imamura, M., Ishizaki, K. (1996). Characterization of p53 gene mutations in esophageal squamous cell carcinoma cell lines: increased frequency and different spectrum of mutations from primary tumors. International journal of cancer.Journal international du cancer 65 ( 3 ): 372-376 . Http://www.ncbi.nlm.nih.gov/pubmed/8575860.
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