HyCyte® | 海星生物

Positive WB detected in：HeLa cells, NIH/3T3 cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells

Positive IP detected in：——

Positive IHC detected in：human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF/ICC detected in：HeLa cells

Positive FC (Intra) detected in：HeLa cells

推荐稀释比：应用 推荐稀释比

Western Blot (WB) WB : 1:10000-1:50000

Immunohistochemistry (IHC) IHC : 1:500-1:2000

Immunofluorescence (IF)/ICC IF/ICC : 1:400-1:1600

Flow Cytometry (FC) (INTRA) FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

文献引用反应性：Human, Mouse, Rat, Monkey, Hamster, Goat

免疫原：CatNo: Ag17503

Product name: Recombinant human ACC protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 2260-2383 aa of BC137287

Sequence: LLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLAEWLEKQLTEEDGVHSVIEENIKCISRDYVLKQIRSLVQANPEVAMDSIIHMTQHISPTQRAEVIRILSTMDSPST

抗体类别：Monoclonal

产品类型：Antibody

全称：acetyl-Coenzyme A carboxylase alpha

计算分子量：2383 aa, 275 kDa

观测分子量：250-270 kDa

GenBank蛋白编号：BC137287

基因名称：Acetyl-CoA Carboxylase 1

Gene ID (NCBI)：31

RRID：AB_2882621

偶联类型：Unconjugated

形式：Liquid

纯化方式：Protein A purification

UNIPROT ID：Q13085

储存缓冲液：PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

储存条件：Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍：ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.

注意事项

1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗等用途。

2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。