HyCyte® | 海星生物

产品说明书下载

取自C57BL/6小鼠脂肪，传代扩增至第五代冻存。本产品经生物安全检测，无细菌、真菌、霉菌、支原体和病毒污染，表达多种小鼠源间质干细胞特异性标记，具有良好的增殖和分化潜能，可分化为成骨细胞、脂肪细胞、软骨细胞等。

细胞名称：HyCyte™ C57BL/6小鼠脂肪间充质干细胞

细胞简称：C57BL/6 ADSC

产品货号：ADMC-C106

种属来源：小鼠，C57BL/6品系

组织来源：脂肪

细胞形态：成纤维细胞样

生长特性：贴壁生长

培养基货号：ADMC-G102

传代比例：1:3-1:6，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

毒素检测：＜10 EU/mL

参考文献：

Zuk PA ,Zhu M ,Mizuno H ,et al. Multilineage cells from human adipose tissue:implications for cell-based therapies.Tissue Eng.2001;7(2):211-228.

Gronthos S, Franklin DM,Leddy HA, et al. Surface proteincharacterization of human adipose tissue-derivedstromal cells. J Ceilular Physiology.2001;189:54-63.

Cousin B, Andre M, Arnaud E, et al. Reconstitution of lethallyirradiated mice by cells isolated from adipose tissue.Biochemical Biophysical Res Communications. 2003,301:1016-1022.

Sen A,Leacurrie YR,Sujkowska D,et al.Adipogenic potentialof human adipose derived stromal cells from multiple donorsis heterogeneous.J Cell Biochem.2001;81:312-319.

Lee JA,Parrett BM,Conejero JA,et al.Biological alchemy-engineering bone and fat from fat-derived stemcells.Ann Plast Surg.2003;50(6):610-710.