HyCyte® | 海星生物

产品说明书下载

取自C57BL/6小鼠骨髓，传代扩增至第五代冻存。本产品经生物安全检测，无细菌、真菌、霉菌、支原体和病毒污染，表达多种小鼠源间质干细胞特异性标记，具有良好的增殖和分化潜能，可分化为成骨细胞、脂肪细胞、软骨细胞等。

细胞名称：HyCyte™ C57BL/6小鼠骨髓间充质干细胞

细胞简称：C57BL/6 MSC

产品货号：BMMC-C106

种属来源：小鼠，C57BL/6品系

组织来源：骨髓

细胞形态：成纤维细胞样

生长特性：贴壁生长

培养基货号：BMMC-G101

传代比例：1:3-1:6，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

毒素检测：＜10 EU/mL

参考文献：

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Encina NR, Billotte WG,Hofmann MC. Immunomagnetic isolation of osteoprogenitors from human bone marrow stroma[J].Lab Invest,1999,79(4):449.

Zohar R, Sodek J,McCulloch CA. Characterization of stronal progenitor cells enriched by flow cytometry[J].Blood, 1997,90(9):3471.

Kicic A,Hall CM, Shen wY,et al.Are stem cell characteristics altered by disease state[JStem Cells Dev.2005,14(1):15.