HyCyte® | 海星生物

产品说明书下载

取自18-45岁成人骨髓，传代扩增至第二代冻存。本产品经生物安全检测，无细菌、真菌、霉菌、支原体和病毒污染，表达多种人源间质干细胞特异性标记，具有良好的增殖和分化潜能，可分化为成骨细胞、脂肪细胞、软骨细胞等。

细胞名称：HyCyte^®成人骨髓间充质干细胞

细胞简称：H MSC

产品货号：BMHX-C106

种属来源：人

组织来源：骨髓

细胞形态：成纤维细胞样

生长特性：贴壁生长

培养基货号：BMHX-G101

传代比例：1:3-1:6，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

毒素检测：＜10 EU/mL

参考文献：

Bartsch T,Falke T , Brehm M. Intra-arterial and intrarnuscular transplanta-tion of adult , autologous bone marrow stem cells. Novel treatment for thera-py refractory peripheral arterial occlusive disease[ J]. Dtsch Med Wochen-schr ,2006 ; 131(3):79-83.

Sohara Y , Shimada H , Minkin C,et al.Bone marrow mesenchymal stemcells provide an alternate pathway of osteoclast activation and bone de-struction by cancer cells[ J]. Cancer Res , 2005 ;65(4):1129-35.

Assali AR , Moustapha A , Sdringola S,et al. Acute coronary syndrome mayoccur with instent restenosis and is associated with adverse outcomes ( thePRESTO trial)[J].Am J Cardiol ,2006;98(6):729-33.

Iso Y, Spees JL,Serrano C,et al.Multipotent human stromal cells improve cardiac function after myocardial infarction in mice without long-term en-graftment[J].Biochem Biophys Res Commun ,2007;354(3):700-6.5

Friendenstein AJ, Chailakhyan RK , Cerasimov Uv ,et al. Bone marrow osteogenic stem cells :in vitro cultivation and transplantation in diffusionchambers[ J ]. Cell Tissue Kinet , 1987;20(3);263-72.