HyCyte® | 海星生物

发货周期：

干冰冻存管运输3天（1x10⁶cells/冻存管x2）

常温运输1-2周发货 (1x10⁶ cells/T25培养瓶）

（推荐T25瓶，复苏发货1-2周）

原价：1500元   套装价特惠价：890元

此细胞株源自BALB/c小鼠由Abelson鼠科白血病病毒诱导的肿瘤。可产生溶菌酶；sIg-,Ia-，Thy-1.2-。为检测到病毒颗粒的分泌，XC斑点形成试验阴性。该细胞可以胞饮中性红并吞噬乳胶颗粒与酵母聚糖；可以经抗体依赖分裂绵羊红细胞与肿瘤靶细胞；LPS或PPD处理2天可诱导分裂红细胞，但对肿瘤靶细胞无作用。

细胞名称：小鼠单核巨噬细胞白血病细胞

细胞简称：RAW 264.7

产品货号：TCM-C766

种属来源：小鼠

组织来源：单核细胞；巨噬细胞

疾病特征：Abelson鼠科白血病病毒诱导的肿瘤

细胞形态：不规则圆形

生长特性：贴壁生长

培养体系：DMEM-H+10%FBS+1%Glutamax+1% Sodium Pyruvate+1%P/S

配套培养基货号：TCM-G766

传代比例：1:3-1:6，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO₂，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

RAW264.7 已发表过的文章：

IF=15.8 Yang X, Fan Y, Liang J, et al. Polyaptamer-Driven Crystallization of Alendronate for Synergistic Osteoporosis Treatment through Osteoclastic Inhibition and Osteogenic Promotion. ACS Nano. 2024;18(33):22431-22443.

IF=14.7 Tong F, Wang Y, Xu Y, Zhou Y, He S, Du Y, Yang W, Lei T, Song Y, Gong T, Gao H. MMP-2-triggered, mitochondria-targeted PROTAC-PDT therapy of breast cancer and brain metastases inhibition. Nat Commun. 2024 Nov 29;15(1):10382. doi: 10.1038/s41467-024-54854-2. PMID: 39613781; PMCID: PMC11607387.

IF=8.2 Li F, Liu T, Liu X, et al. Ganoderma lucidum polysaccharide hydrogel accelerates diabetic wound healing by regulating macrophage polarization[J]. International Journal of Biological Macromolecules, 2024, 260: 129682.

IF=7.9 Che N, Li M, Liu X, et al. Macelignan prevents colorectal cancer metastasis by inhibiting M2 macrophage polarization[J]. Phytomedicine, 2024, 122: 155144.

IF=6.1 Geng R, Zhao Y, Xu W, et al. SIRPB1 regulates inflammatory factor expression in the glioma microenvironment via SYK: functional and bioinformatics insights[J]. Journal of Translational Medicine, 2024, 22(1): 338.

IF=5.6 He S, Yan J, Chen L, et al. Structure and in vitro antioxidant and immunomodulatory activity of a glucan from the leaves of Cyclocarya paliurus[J]. Journal of Functional Foods, 2024, 113: 106016.

IF=5.2 Chen J, Lv Y, Shang W, et al. Loaded delta-hemolysin shapes the properties of Staphylococcus aureus membrane vesicles[J]. Frontiers in Microbiology, 2023, 14: 1254367.

IF=4.8 Chen T, Zhou X, Zhu M, et al. Phytochemical determination and mechanistic investigation of Polygala tenuifolia root (Yuanzhi) extract for bronchitis: UPLC-MS/MS analysis, network pharmacology and in vitro/in vivo evaluation[J]. Journal of Ethnopharmacology, 2024: 118418.

IF=4.6 Yang Y, Tang X, Yao T, et al. Metformin protects ovarian granulosa cells in chemotherapy-induced premature ovarian failure mice through AMPK/PPAR-γ/SIRT1 pathway[J]. Scientific Reports, 2024, 14(1): 1447.

IF=4.6 Jiang J, Zhang D, Liu W, Yang J, Yang F, Liu J, Hu K. Overexpression of NLRP12 enhances macrophage immune response and alleviates herpes simplex keratitis. Front Cell Infect Microbiol. 2024 Jul 25;14:1416105. doi: 10.3389/fcimb.2024.1416105. PMID: 39119293; PMCID: PMC11306119.

IF=4.1 Chen G, Shen L, Hu H, et al. Sulforaphane Inhibits Oxidative Stress and May Exert Anti-Pyroptotic Effects by Modulating NRF2/NLRP3 Signaling Pathway in Mycobacterium tuberculosis-Infected Macrophages[J]. Microorganisms, 2024, 12(6): 1191.

Li W, Peng Y, Liu J, et al. Discovery and Synthesis of Novel Glyrrhizin-Analogs Containing Furanoylpiperazine and the Activity Against Myocardial Injury in Sepsis[J]. Available at SSRN 4821740.

参考文献：

"Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031

Raschke WC, et al. Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198

Denlinger LC, et al. Regulation of inducible nitric oxide synthase expression by macrophage purinoreceptors and calcium. J. Biol. Chem. 271: 337-342, 1996. PubMed: 8550583

Hambleton J, et al. Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages. Proc. Natl. Acad. Sci. USA 93: 2774-2778, 1996. PubMed: 8610116

Taylor GA, et al. Identification of anovel GTPase, the inducibly expresed GTPase, that accumulates in response to interferon gamma. J. Biol. Chem. 271: 20399-20405, 1996. PubMed: 8702776

Li YM, et al. Molecular identity and cellular distribution of advanced glycation endproduct receptors: relationship of p60 to OST-48 and p90 to 80K-H membrane proteins. Proc. Natl. Acad. Sci. USA 93: 11047-11052, 1996. PubMed: 8855306

Panneerselvam K, Freeze HH. Mannose enters mammalian cells using a specific transporter that is insensitive to glucose. J. Biol. Chem. 271: 9417-9421, 1996. PubMed: 8621609

Lokuta MA, et al. Mechanisms of murine RANTES chemokine gene induction by newcatle disease virus. J. Biol. Chem. 271: 13731-13738, 1996. PubMed: 8662857

Taylor MF, et al. In vitro efficacy of morpholino-modified antisense oligomers directed against tumor necrosis factor-alpha mRNA. J. Biol. Chem. 271: 17445-17452, 1996. PubMed: 8663413

Standard Practice for Testing for Biological Responses to Particles in Vitro. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 1903-98R03.

Hartley JW, et al. Expression of infectious murine leukemia viruses by RAW264.7 cells, a potential complication for studies with a widely used mouse macrophage cell line. Retrovirology. 4: 5:1, 2008. PubMed 18177500."

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