HyCyte® | 海星生物

发货周期：

干冰冻存管运输3天（1x10⁶cells/冻存管x2）

常温运输1-2周发货 (1x10⁶ cells/T25培养瓶）

（推荐T25瓶，复苏发货1-2周）

原价：1500元   套装价特惠价：890元

此细胞株源自BALB/c小鼠由Abelson鼠科白血病病毒诱导的肿瘤。可产生溶菌酶；sIg-,Ia-，Thy-1.2-。为检测到病毒颗粒的分泌，XC斑点形成试验阴性。该细胞可以胞饮中性红并吞噬乳胶颗粒与酵母聚糖；可以经抗体依赖分裂绵羊红细胞与肿瘤靶细胞；LPS或PPD处理2天可诱导分裂红细胞，但对肿瘤靶细胞无作用。

细胞名称：小鼠单核巨噬细胞白血病细胞【STR鉴定报告可咨询获取】

细胞简称：RAW 264.7

产品货号：TCM-C766

种属来源：小鼠

组织来源：单核细胞；巨噬细胞

疾病特征：Abelson鼠科白血病病毒诱导的肿瘤

细胞形态：不规则圆形

生长特性：贴壁生长

培养体系：DMEM-H+10%FBS+1%Glutamax+1% Sodium Pyruvate+1%P/S

配套培养基货号：TCM-G766

传代比例：1:3-1:6，每2-3天换液一次

倍增时间：48-72 h

培养条件：气相：95%空气+5%CO₂，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

RAW264.7 已发表过的文章：

IF=15.7 Jin H, Zhu X, Zhang M, Zheng H, Chen Y, Yao D, Liu S, Zhang H. Cu/Zn/histidine supramolecular assemblies with optimized Cu catalytic sites as an alternative of superoxide dismutase. Nat Commun. 2025 Nov 18;16(1):10075.

IF=15.8 Yang X, Fan Y, Liang J, et al.Polyaptamer-driven crystallization of alendronate for synergistic osteoporosistreatment through osteoclastic inhibition and osteogenic promotion[J]. ACSnano, 2024, 18(33): 22431-22443.

IF=14.7 Tong F, Wang Y, Xu Y, et al.MMP-2-triggered, mitochondria-targeted PROTAC-PDT therapy of breast cancer andbrain metastases inhibition[J]. Nature Communications, 2024, 15(1): 10382.

IF=14.1 Zheng D, Bai Y, Liu Z, Zhao Z, Kong Y, Xu Y, Jia Q, Zhu R, Hu Y. Immunoaffinity-Mimetic Assembly of Peptide-Aptamer Conjugates and Stem Cell-Derived Exosomes into Hierarchical Microgels for Spinal Cord Injury Repair. Adv Sci (Weinh). 2026 Jan 9:e19701.

IF=11.3 Wang J, Liu H, Pan X, Liu T, Li Q, Yuan Y, Zhang J, Zhao S, Yan X, Su J. Daphnetin alleviates influenza pneumonia by enhancing chaperone-mediated autophagy of KEAP1 through Heat shock cognate 71 kDa protein. Phytomedicine. 2026 Jun 29;159:158517.

IF=8.2 Li F, Liu T, Liu X, et al. Ganodermalucidum polysaccharide hydrogel accelerates diabetic wound healing byregulating macrophage polarization[J]. International Journal of BiologicalMacromolecules, 2024, 260: 129682.

IF=7.9 Che N, Li M, Liu X, et al.Macelignan prevents colorectal cancer metastasis by inhibiting M2 macrophagepolarization[J]. Phytomedicine, 2024, 122: 155144.

IF=7.9 Hongpeng Duan, Zhiyuan Chen, Xinyue Wang, Bei Zhang, Liangbo Guo, Wenhao Lv, Zhenbin Xu, Shaosheng Wu, Fan Yu, Difan Wang, Weijing Zhang, Yifan Lian, Shengmin Zhang,Biomimetic nanoplatform potentiates STING-immunotherapy by coordinated delivery of chemotherapeutic and innate immune agonist,Materials & Design,Volume 261,2026,115372.

IF=7.7 Yang K, Yang Y, Si L, et al. Seeingis believing: Facile amphiphilic AIEgen unveils selective and ultrafastwash-free dead cell imaging[J]. Sensors and Actuators B: Chemical, 2025, 430:137383.

IF=6.1 Geng R, Zhao Y, Xu W, et al. SIRPB1regulates inflammatory factor expression in the glioma microenvironment viaSYK: functional and bioinformatics insights[J]. Journal of TranslationalMedicine, 2024, 22(1): 338.

IF=5.6 He S, Yan J, Chen L, et al.Structure and in vitro antioxidant and immunomodulatory activity of a glucanfrom the leaves of Cyclocarya paliurus[J]. Journal of Functional Foods, 2024,113: 106016.

IF=5.2 Chen J, Lv Y, Shang W, et al. Loadeddelta-hemolysin shapes the properties of Staphylococcus aureus membranevesicles[J]. Frontiers in Microbiology, 2023, 14: 1254367.

IF=5.2 Jingjing Han, Bolin Zhu, Qin Xu, Xiaoning Li, Long Cao, Fugui Guo, Xiaomin Niu, Hongyan Ji, Man Li, Guoshun Zhang, Liwen Han, Shiping Yu, Shuqiu Zhang, Guolian Ren,Integrated development and evaluation of a gel delivery system for Sophora flavescens extract: from optimized extraction to multifunctional topical efficacy,Journal of Drug Delivery Science and Technology,Volume 124,2026,108634.

IF=4.8 Chen T, Zhou X, Zhu M, et al.Phytochemical determination and mechanistic investigation of Polygalatenuifolia root (Yuanzhi) extract for bronchitis: UPLC-MS/MS analysis, networkpharmacology and in vitro/in vivo evaluation[J]. Journal of Ethnopharmacology,2024: 118418.

IF=4.6 Yang Y, Tang X, Yao T, et al.Metformin protects ovarian granulosa cells in chemotherapy-induced prematureovarian failure mice through AMPK/PPAR-γ/SIRT1 pathway[J]. Scientific Reports,2024, 14(1): 1447.

IF=4.6 Jiang J, Zhang D, Liu W, et al.Overexpression of NLRP12 enhances macrophage immune response and alleviatesherpes simplex keratitis[J]. Frontiers in Cellular and Infection Microbiology,2024, 14: 1416105.

IF=4.1 Chen G, Shen L, Hu H, et al.Sulforaphane Inhibits Oxidative Stress and May Exert Anti-Pyroptotic Effects byModulating NRF2/NLRP3 Signaling Pathway in Mycobacterium tuberculosis-InfectedMacrophages[J]. Microorganisms, 2024, 12(6): 1191.

IF=4.7 Li W, Peng Y, Liu J, et al.Discovery and synthesis of novel glyrrhizin-analogs containingfuranoylpiperazine and the activity against myocardial injury in sepsis[J].Bioorganic Chemistry, 2024, 153: 107846.

参考文献：

Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031

Raschke WC, et al. Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198

Denlinger LC, et al. Regulation of inducible nitric oxide synthase expression by macrophage purinoreceptors and calcium. J. Biol. Chem. 271: 337-342, 1996. PubMed: 8550583

Hambleton J, et al. Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages. Proc. Natl. Acad. Sci. USA 93: 2774-2778, 1996. PubMed: 8610116

Taylor GA, et al. Identification of anovel GTPase, the inducibly expresed GTPase, that accumulates in response to interferon gamma. J. Biol. Chem. 271: 20399-20405, 1996. PubMed: 8702776

Li YM, et al. Molecular identity and cellular distribution of advanced glycation endproduct receptors: relationship of p60 to OST-48 and p90 to 80K-H membrane proteins. Proc. Natl. Acad. Sci. USA 93: 11047-11052, 1996. PubMed: 8855306

Panneerselvam K, Freeze HH. Mannose enters mammalian cells using a specific transporter that is insensitive to glucose. J. Biol. Chem. 271: 9417-9421, 1996. PubMed: 8621609

Lokuta MA, et al. Mechanisms of murine RANTES chemokine gene induction by newcatle disease virus. J. Biol. Chem. 271: 13731-13738, 1996. PubMed: 8662857

Taylor MF, et al. In vitro efficacy of morpholino-modified antisense oligomers directed against tumor necrosis factor-alpha mRNA. J. Biol. Chem. 271: 17445-17452, 1996. PubMed: 8663413

Standard Practice for Testing for Biological Responses to Particles in Vitro. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 1903-98R03.

Hartley JW, et al. Expression of infectious murine leukemia viruses by RAW264.7 cells, a potential complication for studies with a widely used mouse macrophage cell line. Retrovirology. 4: 5:1, 2008. PubMed 18177500.

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