HyCyte cell line: HyCyte C-33 A stable cell line for hGALNT2 overexpression

Catalogue No.: CGSC-M32951

Gene: hGALNT2

Gene ID: 2590

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：1

Target transcript: NM_004481.5

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：C-33 A

Species：Human

Passage：5

Doubling time：36--48h

Growth properties: Adherent

Recommended culture media:Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G143).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).