HyCyte cell line: HyCyte B16-F10#Itgb3 mSrc_p.E99A_c.296A＞C cell line

Catalogue No.:   CGMU-M2276

Parental:   B16-F10#Itgb3

Gene:   mSrc

Gene ID:   20779

Genotype：Homozygous

Mutation：p.E99A_c.296A＞C

Species：mouse

Passage: 9

Doubling time: 18--24h

Growth Properties：Adherent

Recommended culture media:   Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCM-G713).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).