HyCyte Cell Line: HyCyte HCT116-Luc KO-hCLCA1

Parental: HCT116-Luc

Catalogue No.: CGKO-M2927

Species: Human

Gene: hCLCA1

Gene ID: 1179

Passage: 9

Doubling time: 48-72 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

McCoy's 5A Medium supplemented with 10 % FBS, 1.0 ug/mL Puromycin, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G185L).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:8.

Freezing media: McCoy's 5A + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201)

Genotype: hCLCA1 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative