RH-35 cells originate from transplantable Reuber H-35 hepatoma induced by N-2-fluorenyldiacetamide in male AxC rats. RH-35 cells are small, and the provider states that 24-hour starvation can synchronize them.

Cell Name: Rat Hepatoma Cells

Cell Synonyms: RH-35; H4IIEC3; H4-IIE-C3; H4-II-EC3; Reuber-H-35 hepatoma; Reuber H-35; Reuber H35; H-35 Reuber; H35 Reuber; RH-35; RH35; H35; H-II-E-C3

Catalogue No.: TCR-C630

Species: Rat

Tissue Origin: Hepatoma

Disease Characteristics: Hepatobiliary Cancer Cells

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCR-G630

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: —

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection