NRK-52E is a cell line with an epithelial morphology, isolated from the rat kidney.

Cell Name: Normal rat kidney epithelial cell line

Cell Synonyms: NRK-52E; NRK 52E; NRK52E; NRK clone 52E; Normal Rat Kidney-52E; NRK-E52

Catalogue No.: TCR-C629
Species: Rat
Tissue Origin: Kidney

Disease Characteristics: —

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 5% FBS+ 1% P/S
Matching Medium Catalog Number: TCR-G629

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 45 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection