Cortisol can inhibit the growth of these cells. These cells express antigens specific to intestinal epithelium, maintaining a constant growth rate and cell morphology up to passage 20, after which the growth rate rapidly declines and the cell morphology also changes.

Cell Name: Rat small intestinal epithelial cell line

Cell Synonyms: IEC-6; IEC 6; IEC6; Intestinal Epithelioid Cell line No. 6

Catalogue No.: TCR-C626
Species: Rat
Tissue Origin: Small Intestine
Disease Characteristics: —
Morphology: Epithelial-like
Growth Properties: Adherent
Culture System: DMEM-H + 10% FBS + 0.01 mg/mL bovine insulin + 1% P/S

Matching Medium Catalog Number: TCR-G626

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection