This cell line is derived from a transplantable adrenal pheochromocytoma of a male rat; it expresses nerve growth factor (NGF) receptors, and NGF can induce the cells to develop a reversible neuronal phenotype. The cells are capable of producing catecholamines, dopamine, and norepinephrine, but do not synthesize epinephrine.

Cell Name: Rat adrenal pheochromocytoma cell line

Cell Synonyms: PC12; PC-12; PC 12

Catalogue No.: TCR-C617
Species: Rat
Tissue Origin: Adrenal Gland
Disease Characteristic: Pheochromocytoma
Morphology: Small, irregularly shaped cells
Growth Properties: Suspended and clustered growth, with a small number of loosely adherent cells

Culture Medium: RPMI-1640 + 5% FBS + 10% HS + 1% P/S
Matching Medium Catalog Number: TCR-G617

Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection