This cell line is derived from a transplantable male rat adrenal pheochromocytoma. These cells express nerve growth factor (NGF) receptors. NGF can induce the development of a neuronal phenotype. These cells do not synthesize adrenaline.

Cell Name: Rat Adrenal Pheochromocytoma Cells (Highly Differentiated)

Cell Synonyms: PC-12

Catalogue No.: TCR-C616

Species : Rat
Tissue Origin: Adrenal gland

Disease Characteristics: Adrenal pheochromocytoma

Morphology: Polygonal, spindle-shaped

Growth Properties: Adherent growth
Culture Medium: RPMI-1640 + 10% FBS (fetal bovine serum) + 1% P/S (penicillin-streptomycin)
Passage Ratio: 1:2–1:4; change medium every 2–3 days
Doubling Time: 36–48 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Recommended HyCyte® One-Step Cryopreservation Medium (Ready-to-use, serum-free, no programmed cooling required): GUCP-R201

Quality Control: Negative for bacteria, fungi, and mycoplasma.