NR8383 (Normal rat, 1983) was derived from normal rat alveolar macrophages obtained during lung lavage. The cells were cultured for approximately 8–9 months in the presence of continuous cultures of gerbil lung cells. Subsequently, exogenous growth factors were no longer required. The NR8383 cell line was cloned and subcloned from single cells via limiting dilution and further subcloned three times using soft agar. The cells exhibit macrophage characteristics, including phagocytosis of zymosan and Pseudomonas aeruginosa, nonspecific esterase activity, Fc receptors, and oxidative degradation. They secrete IL-1, TGF-β, and IL-6 and reproducibly respond to exogenous growth factors. NR8383 cells respond to bleomycin by secreting TGF-β precursor. Under bleomycin stimulation, TGF-β mRNA expression is also upregulated. The cells are sensitive to endotoxin. LPS levels of 1–10 ng/mL inhibit proliferation by 50%. Even at levels as high as 0.001 mg/mL, LPS inhibition remains nontoxic and reversible in subsequent processes. The NR8383 cell line provides a highly responsive, homogeneous source of alveolar macrophages suitable for in vitro studies of macrophage-related activities.
Cell Name: Rat alveolar macrophage cell line
Cell Synonyms:NR8383; NR-8383; NR 8383; NR8383.1; AgC11x3A; Normal Rat, August 3, 1983
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days Doubling Time: 97-120 h Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201 Quality Control: Negative for bacteria, fungi, and mycoplasma detection
Key Points for NR8383 Cell Culture:
After resuscitation, cell proliferation is slow and typically requires around 7 days. Replace the medium once after 3 days.
Cells should be cryopreserved at a higher density, recommended at 2-3×10⁶ cells/mL.
NR8383 cells are sensitive to endotoxins. It is advised to use reagents and disposable cell culture consumables with low endotoxin levels during cultivation.
Variations in serum quality may lead to fluctuations in doubling time. High-quality fetal bovine serum is recommended.