L6 myoblasts were initially isolated by Yaffe from the first two passages of primary cultures of rat thigh muscle in the presence of methylcholanthrene. L6 cells can fuse in culture medium to form multinucleated myotubes and striated muscle fibers. The degree of fusion of L6 cells decreases with increasing passage number; therefore, L6 cells should be cryopreserved at low passage and periodically recloned to select for cells with strong fusion capability. If the cells in the culture vessel are allowed to reach confluence, the myogenic component of L6 cells will rapidly deplete.

Cell Name: Rat myoblast cell line

Cell Synonyms: L6; L-6; L-6 myoblast

Catalogue No.:TCR-C609
Species: Rat
Tissue Origin: Skeletal Muscle
Disease Characteristics: —
Morphology: Myoblast-like

Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCR-G609

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection