This cell line is derived from X-ray-irradiated transplanted insulinoma in rats. It is insulin-positive and capable of synthesizing proinsulin I and II, making it suitable for beta-cell function research.

Cell Name: Rat insulinoma cell line

Cell Synonyms: INS-1; INS1

Catalogue No.: TCR-C608

Species: Rat
Tissue Origin: Pancreas
Disease Characteristic: Insulinoma cells
Morphology: Irregular polygonal cells
Growth Characteristics: Adherent growth
Culture System: RPMI-1640 + 10% FBS + 50 μmol/L β-mercaptoethanol + 1% P/S
Matching Medium No.: TCR-G608
Passage Ratio: 1:3–1:6; change medium every 2–3 days
Doubling Time: 48–72 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma