Product Name:Luciferase Reporter C6 Cell Line
HyCyte cell line:HyCyte C6-Luc
Catalog No.:TCR-C605L
Construction method:Lentivirus
Selection Marker:Puromycin
Parental:C6
Species:Rat
Morphology:Fibroblast-like
Growth properties:Suspension
Culture Medium:Ham's F-12K supplemented with 15 % HS,16.0 ug/mL Puromycin, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCR-G605L).
Passage:1:2-1:6
Doubling time:48-72 h
Subculture:Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:6.
Freezing media:Ham's F-12K+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).