C6 is a clone of rat glioma induced by N-nitrosourea (specifically N-methyl-N-nitrosourea) by Benda et al., and was established after a series of alternating in vitro culture and animal passage procedures. This cell line expresses S-100; produces growth hormone; and can produce phosphoglycerate dehydrogenase under the action of glucocorticoids. When the cells grow from low density to confluence, the production of S-100 increases tenfold.

Cell Name: Rat glioma cell line

Cell Synonyms: C6; C-6; C 6; RGC-6; RGC6; RGc6

Catalogue No.: TCR-C605

Species: Rat
Tissue Origin: Brain
Disease Feature: Brain Glioma
Cell Morphology: Fibroblast-like
Growth Characteristics: Adherent growth
Culture System: F12K + 2.5% FBS + 15% HS + 1% P/S
Matching Medium No.: TCR-G605
Passage Ratio: 1:2–1:6; change medium every 2–3 days
Doubling Time: 48–72 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂; Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO; stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma