The serum-free conditioned medium of BRL 3A cells is a source of MSA, and the MSA peptide family can partially meet the serum requirements of chicken embryo fibroblasts.

Cell Name: Buffalo rat liver cell line

Cell Synonyms: BRL 3A; BRL3A; BRL 3A; Buffalo Rat Liver-3A

Catalogue No.: TCR-C604
Species: Rat

Tissue Origin: Liver

Disease Characteristics: —

Morphology: Fibroblast-like

Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCR-G604

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection